Journal article
The balance of interleukin-12 and interleukin-23 determines the bias of MAIT1 versus MAIT17 responses during bacterial infection
H Wang, AG Nelson, B Wang, Z Zhao, XY Lim, M Shi, LJ Meehan, X Jia, K Kedzierska, BS Meehan, SBG Eckle, MNT Souter, TJ Pediongco, JYW Mak, DP Fairlie, J McCluskey, Z Wang, AJ Corbett, Z Chen
Immunology and Cell Biology | Published : 2022
DOI: 10.1111/imcb.12556
Abstract
Mucosal-associated invariant T (MAIT) cells are a major subset of innate-like T cells mediating protection against bacterial infection through recognition of microbial metabolites derived from riboflavin biosynthesis. Mouse MAIT cells egress from the thymus as two main subpopulations with distinct functions, namely, T-bet-expressing MAIT1 and RORγt-expressing MAIT17 cells. Previously, we reported that inducible T-cell costimulator and interleukin (IL)-23 provide essential signals for optimal MHC-related protein 1 (MR1)-dependent activation and expansion of MAIT17 cells in vivo. Here, in a model of tularemia, in which MAIT1 responses predominate, we demonstrate that IL-12 and IL-23 promote MA..
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Grants
Awarded by Guangzhou Medical University
Funding Acknowledgements
We thank B Becher, University of Zurich, Institute of Experimental Immunology, for provision of the IL-23-Ig construct, and V Kuchroo, Harvard Medical School and Brigham and Women's Hospital, for the IL-23R-GFP reporter mice. We thank H Newton for provision of L.longbeachae NSW150, I. Van Driel (Bio21 Institute) for provision of Il-12p35<SUP>-/-</SUP> and Il-12p40<SUP>-/-</SUP> mice and Gavin Painter (Ferrier Research Institute, Victoria University of Wellington, New Zealand) for supply of 5-amino-6-d-ribitylaminouracil (5-A-RU) used in the generation of MR1-5-OP-RU-tetramers. We thank Tina Luke and the staff at the Doherty Institute node of the Melbourne Cytometry Platform, and the staff at the Biological Research Facility of the Doherty Institute for facility access and technical assistance. We acknowledge the following funding: National Health and Medical Research Council (NHMRC) of Australia Program Grant 1113293 (JMc), NHMRC Investigator Grants 1193745 (AJC), 1196881 (SBGE), 2009551 (DPF) and 1173871 (KK), NHMRC Project Grant 1120467 (AJC, JMc, ZC) and NHMRC SPR Fellowship 1117017 (DPF), National Natural Science Foundation of China Young Scientists Fund 82001686 (HW), National Institute of Allergy and Infectious Diseases of the National Institutes of Health (NIH) Grant R01AI148407 (JMc, DPF), Australian Research Council (ARC) Future Fellowship FT160100083 (AJC), ARC Centre of Excellence in Advanced Molecular Imaging CE140100011 (DPF), The University of Melbourne Dame Kate Campbell Fellowships (AJC, KK), Guangzhou Medical University Nanshan Fellowship (HW). Open access publishing facilitated by The University of Melbourne, as part of the Wiley - The University of Melbourne agreement via the Council of Australian University Librarians.